Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TP63

Cell type

Cell type Class
Digestive tract
Cell type
KYSE-180
Primary Tissue
Esophagus
Tissue Diagnosis
Carcinoma Squamous Cell

Attributes by original data submitter

Sample

source_name
Esophageal squamous cell carcinoma
cell line
ESCC cell line KYSE180
chip antibody
p63 (Santa Cruz, sc-8431)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked using 1% formaldehyde for 20 minutes. Subsequently, fixed cells were lysed, sheared and subjected to immunoprecipitation with respective antibodies. The immunocomplexes were captured and the DNA extraction was done using phenol/chloroform/isoamyl. ChIP-seq libraries were constructed using the KAPA HyperPrep kit according to the manufacturer's manual.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
9364454
Reads aligned (%)
97.5
Duplicates removed (%)
10.3
Number of peaks
31043 (qval < 1E-05)

hg19

Number of total reads
9364454
Reads aligned (%)
97.0
Duplicates removed (%)
10.8
Number of peaks
31022 (qval < 1E-05)

Base call quality data from DBCLS SRA